foxp3 fix perm kit Search Results


foxp3 perm/fix kit  (Thermo Fisher)
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Thermo Fisher foxp3 perm/fix kit
Foxp3 Perm/Fix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flowx foxp3 transcription factor fixation perm buffer kit  (R&D Systems)
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R&D Systems flowx foxp3 transcription factor fixation perm buffer kit
Flowx Foxp3 Transcription Factor Fixation Perm Buffer Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fix/perm buffer (foxp3/transcription factor staining kit, ebioscience)  (Thermo Fisher)
90
Thermo Fisher fix/perm buffer (foxp3/transcription factor staining kit, ebioscience)
Fix/Perm Buffer (Foxp3/Transcription Factor Staining Kit, Ebioscience), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-mouse foxp3 staining kit  (Thermo Fisher)
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Thermo Fisher anti-mouse foxp3 staining kit
Anti Mouse Foxp3 Staining Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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perm solution  (Cytek Biosciences)
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Cytek Biosciences perm solution
Perm Solution, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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foxp3 fix perm kit  (Cytek Biosciences)
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Cytek Biosciences foxp3 fix perm kit
Foxp3 Fix Perm Kit, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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foxp3 fix/perm kit  (Thermo Fisher)
90
Thermo Fisher foxp3 fix/perm kit
NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and <t>Foxp3.</t> Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).
Foxp3 Fix/Perm Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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foxp3/transcription factor fix/perm kit  (Thermo Fisher)
90
Thermo Fisher foxp3/transcription factor fix/perm kit
NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and <t>Foxp3.</t> Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).
Foxp3/Transcription Factor Fix/Perm Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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foxp3 fix permeabilisation kit  (Thermo Fisher)
86
Thermo Fisher foxp3 fix permeabilisation kit
NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and <t>Foxp3.</t> Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).
Foxp3 Fix Permeabilisation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fix/perm buffer  (Thermo Fisher)
90
Thermo Fisher fix/perm buffer
NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and <t>Foxp3.</t> Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).
Fix/Perm Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fix/perm buffer/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
fix/perm buffer - by Bioz Stars, 2026-02
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perm kit  (Cytek Biosciences)
94
Cytek Biosciences perm kit
NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and <t>Foxp3.</t> Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).
Perm Kit, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/perm kit/product/Cytek Biosciences
Average 94 stars, based on 1 article reviews
perm kit - by Bioz Stars, 2026-02
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Image Search Results


NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and Foxp3. Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).

Journal: Frontiers in Immunology

Article Title: NK1.1 Expression Defines a Population of CD4 + Effector T Cells Displaying Th1 and Tfh Cell Properties That Support Early Antibody Production During Plasmodium yoelii Infection

doi: 10.3389/fimmu.2018.02277

Figure Lengend Snippet: NK1.1 + CD4 + T cells express Tbet and Bcl6 and produce effector cytokines. (A) Representative intracellular staining of NK1.1 − and NK1.1 + CD4 + T cells for the transcription factors Tbet, Bcl6, RORγt, Gata3, and Foxp3. Cells previously gated on live, CD44 hi CD62L lo CD4 + TCRβ + splenocytes. Gates based on FMO controls shown in Supplemental Figure . (B) Frequency of NK1.1 + and NK1.1 − CD4 + T cells expressing Tbet, Bcl6 or, Tbet and Bcl6 on days 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01. (C) Median fluorescence intensity (MFI) for Tbet and Bcl6 in NK1.1 + and NK1.1 − CD4 + T cells on day 8 and 11 p.i. An aligned rank transformation was performed on non-parametric data before determining significance by two-way ANOVA with a post hoc Holm-Sidak's multiple comparisons test. * p < 0.05. (D) Representative intracellular cytokine staining of NK1.1 − and NK1.1 + CD4 + T cells previously gated on live CD4 + TCRβ + T cells, on day 7 post-infection. Splenocytes were stimulated for 4 h with PMA and ionomycin in the presence of brefeldin A before being stained for IFN-γ, IL-21, and TNFα. (E) The frequency of single, double, or triple cytokine-producing cells at day 7 post-infection. Significance assessed via an unpaired non-parametric Mann-Whitney test. * p < 0.05. Data are representative of three independent experiments (error bars, s.e.m.).

Article Snippet: For nuclear transcription factor and Ki-67 staining, cells were first surface stained as described, followed by simultaneous fixation and permeabilization with a FoxP3 fix/perm kit per manufacturers direction (ThermoFisher Scientific).

Techniques: Staining, Expressing, Transformation Assay, Fluorescence, Infection, MANN-WHITNEY